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鼠标丸器官中的精子生成的ex vivo时空特征

Jiachen Sun¹, Lifa Zhu¹, Yutong Li¹

¹State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu, 211166, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|October 31, 2025

概括

新的丸有机体 (TOs) 从新生小鼠丸中成功自我重建,支持体外精子生成并产生 haploid 精子. 这一突破为生育保护研究提供了一个有希望的平台.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
干细胞科学干细胞科学

背景情况:

目前的丸器官 (TO) 协议面临着结构完整性,介质性错误和不完整的体外精子生成 (IVS) 的挑战.
保持生育能力和研究丸发育需要强大的体外模型.

研究的目的:

从新生小鼠丸制造功能丸器官的新战略.
评估这些有机体的自我复合,生殖细胞分化和体外精子生成的能力.

主要方法:

从新生儿小鼠丸中单细胞悬浮物的自我复合到TOs.
形态分析和谱系特定标记物的评估.
单细胞RNA测序 (scRNA-seq) 用于分析细胞群和分化阶段.

主要成果:

开发的TOs成功地概述了丸结构和精子生成的关键方面,包括管状形成和生殖细胞分化.
器官体表现出适当的介质事件,并衍生出类似于体内四/五阶段圆精子细胞的单体细胞.
化学TOs,即使具有多样化的遗传背景,也支持胚胎细胞发展成单 haploid 精子.

结论:

新的TO平台展示了自我修复能力,并支持体外精子生成的先进阶段.
这些发现为研究丸微环境,体外精子生成和生育保护策略带来了重大进展.

关键词:

在体外的精子生成.不孕不育不孕不育不孕不育介质变化 (meiosis) 是一种变质的过程.丸的有机体丸的有机体

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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...