相关实验视频

Updated: Jan 12, 2026

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

在SLE中探索内皮功能障碍:通过dsDNA激活cGAS-STING-IRF3通路

Zhou Yan¹, Li Li², Zha Shun³

¹Department of Nephrology, The First People's Hospital of Yunnan Province /The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.

|November 1, 2025

概括

细胞外双链DNA (dsDNA) 通过激活cGAS-STING-IRF3通路,直接损害内皮细胞,导致系统性红斑狼 (SLE) 的血管损伤. 针对这种途径可能会提供新的SLE治疗方法.

科学领域:

免疫学免疫学免疫学
血管生物学血管生物学
分子医学是分子医学.

背景情况:

系统性红斑狼 (SLE) 与抗双链DNA (抗dsDNA) 抗体和内皮功能障碍有关.
在SLE中,连接细胞外DNA与血管损伤的精确机制尚未完全理解.

研究的目的:

研究细胞外双链DNA (dsDNA) 对内皮细胞的直接影响.
为了阐明参与dSDNA诱导的内皮损伤的分子途径.
为了将这些发现与SLE患者的内皮功能障碍标志物相关联.

主要方法:

从SLE患者和健康对照组收集的临床样本.
使用人类静脉内皮细胞 (HUVEC) 进行体外研究.
采用细胞活力测试,ELISA,RT-qPCR,西斑和免疫光检测来评估内皮损伤和信号通路.

主要成果:

患有SLE的患者,特别是那些具有抗dsDNA抗体的患者,表现出威尔布兰德因子 (vWF),可溶性血栓模块素 (sTM) 和E-selectin的血清水平升高.
在体外,dDNA暴露降低了HUVEC活力,并提高了vWF,sTM和E-selectin的分泌.
dsDNA激活了内皮细胞中的cGAS-STING-IRF3信号通路,由增加的基因和蛋白质表达和增强的cGAS定位证实.

结论:

没有细胞的dsDNA通过cGAS-STING-IRF3通路直接诱导内皮功能障碍.
这种机制有助于在SLE中观察到的血管损伤.
cGAS-STING-IRF3轴为SLE和相关疾病提供了潜在的治疗目标.

关键词:

系统性红血性狼 (Systemic lupus erythematosusus) 是一种全身性狼.cGAS-STING-IRF3通道中的一个.dsDNADNADNA 的意思是什么血管内皮损伤血管内皮损伤

更多相关视频

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Published on: June 16, 2020

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Published on: March 26, 2019

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...