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细胞外RNA作为一个分子驱动器和治疗目标在腹腔大动脉动脉瘤

Nahla Ibrahim¹, Hubert Hayden¹, Gabriel Kurzreiter¹

¹Division of Vascular Surgery, Department of General Surgery, Medical University of Vienna and University Hospital Vienna, Vienna, Austria.

Scientific reports

|November 1, 2025

概括

细胞外RNA (exRNA) 降解,而不是exDNA,小鼠的腹腔大动脉动脉瘤 (AAA) 增长减弱. 系统性RNaseA治疗减少了炎症并保持了血管完整性,为AAA疾病提供了一种新的治疗策略.

科学领域:

心血管生物学心血管生物学
血管医学血管医学
分子生物学分子生物学

背景情况:

腹腔大动脉瘤 (AAA) 涉及炎症,矩阵降解和光滑肌肉细胞 (SMC) 损失,目前没有药物治疗.
细胞外核酸 (exNA) 与心血管炎症和组织损伤有关.

研究的目的:

研究细胞外DNA (exDNA) 和细胞外RNA (exRNA) 在AAA病变发生过程中的作用.
评估exDNA和exRNA作为已确定的AAA疾病中的潜在治疗点.

主要方法:

在AAA患者和小鼠模型中分析循环的exDNA和exRNA水平.
在已确定的AAA.小鼠中,给RNase A (降解exRNA) 或DNase I (降解exDNA) 用.
对动脉瘤生长,全身炎症和血管组织重塑的评估.

主要成果:

在AAA患者和小鼠模型中,循环 exDNA 升高; exRNA 水平与AAA 发展无关.
使用RNase A显著减弱小鼠的AAA生长,对exRNA的向降解.
RNase A治疗减少了全身炎症并保持了SMC完整性,而DNase I没有显著的影响.

结论:

循环中的exDNA可以作为AAA的潜在生物标志物.
用系统性RNase A向exRNA代表了一种有前途的治疗策略,通过调节炎症和组织重塑来缓解AAA进展.

关键词:

腹腔大动脉动脉瘤腹腔大动脉动脉瘤细胞自由的DNA 细胞自由的DNA 没有细胞的RNA.细胞外DNA是细胞外DNA.细胞外RNA是细胞外RNA.鼠标模型鼠标模型

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