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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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相关实验视频

Updated: Jan 12, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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LncRNA:使用LongTarget及其修订版本进行DNA结合预测.

Jie Lin1,2, Yujian Wen1, Hai Zhang3

  • 1Bioinformatics Section, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Methods in molecular biology (Clifton, N.J.)
|November 1, 2025
PubMed
概括

预测长非编码RNA (lncRNA) DNA结合部位对于理解基因调节至关重要. 本研究详细介绍了LongTarget和Fasim-LongTarget工具,用于准确的计算预测lncRNA:DNA相互作用.

关键词:
表观遗传调节 表观遗传调节长距离的长距离目标长时间目标长时间目标.这里是三重楼.在cnRNA中.在cRNA:DNA结合.

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相关实验视频

Last Updated: Jan 12, 2026

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 长非编码RNAs (lncRNAs) 通过与DNA结合来调节基因表达.
  • 对lncRNA:DNA结合位点 (DBS) 的实验性识别是全基因组的挑战.
  • 对lncRNA:DNA相互作用的计算预测至关重要.

研究的目的:

  • 描述LongTarget和Fasim-LongTarget的逐步使用,用于预测DNA结合域 (DBD) 和DBS.
  • 介绍LongMan数据库,用于探索哺乳动物的lncRNA及其DNA结合.
  • 使用公共数据证明预测的DBD和DBS的评估.

主要方法:

  • 使用LongTarget和Fasim-LongTarget软件进行计算预测.
  • 使用LongMan数据库对lncRNAs进行比较基因组学.
  • 应用UCSC基因组浏览器用于验证预测的结合位点.

主要成果:

  • 提供了使用LongTarget和Fasim-LongTarget的详细协议.
  • 通过LongMan. 实现了对特定物种的lncRNA及其DNA相互作用的探索.
  • 对预测的DBD和DBS进行有效的评估策略.

结论:

  • 长标和Fasim-LongTarget提供了有效的计算工具来预测lncRNA:DNA结合.
  • 长人数据库有助于对lncRNA功能进行跨物种分析.
  • 准确的预测和评估方法是理解 lncRNA 中介基因调节的关键.