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Updated: Jan 12, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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一个基于gasdermin的生死进化系统,用于重新编程蛋白酶特异性

Ziqi Gao1, Tianzhen Li1, Hao Ye1

  • 1Department of Chemistry, Research Center for Chemical Biology and Omics Analysis, Guangdong Provincial Key Laboratory of Catalysis, College of Science, Southern University of Science and Technology, Shenzhen, China.

Nature chemical biology
|November 1, 2025
PubMed
概括

研究人员开发了一种用于重新编程蛋白酶特异性的新系统. 这种方法使用有毒蛋白标记物有效地选择工程蛋白酶,使蛋白质编辑和治疗中的应用成为可能.

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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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