酒酵母消耗β-葡萄糖的微波酶级联脱聚合:从分支对分支的结构洞察到功能性寡糖类生产
Guozheng Wei1, Wenqi Shang1, Guohua Zhao2
1Yibin Academy of Southwest University, Yibin 644000, China; College of Food Science, Southwest University, Chongqing 400715, China.
Food research international (Ottawa, Ont.)
|November 1, 2025
概括
酒的消耗酵母可以通过微波水解和酶处理的组合转化为有价值的生物活性寡糖. 这种可持续的方法将酒厂的废物转化为循环经济的功能性碳水化合物.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 生物化学 生化学
背景情况:
- 酒消耗酵母 (BSY) 是酒生产的主要副产品.
- BSY含有大量β-葡萄糖,是一种有价值的多糖.
- 目前用于β-葡萄糖提取和利用的方法是有限的.
研究的目的:
- 从BSYβ-葡萄糖中提取生物活性寡糖的综合方法.
- 为了描述所获得的寡糖的结构和特性.
- 在循环经济框架内探索BSY价值化潜力.
主要方法:
- 用酸介导的微波水解BSYβ-葡萄糖.
- 使用一种新型β-1,6-特异性内分葡萄糖酶 (Gly30z) 的酶性加工.
- 使用染色学和酶学方法对寡糖类产品进行表征.
主要成果:
- 优化的微波水解产生了高效率的β-1,3结合的寡糖化物 (DP2-5).
- Gly30z酶精确地切断了β-1,6-糖化链接,产生了仁和特定的三糖化物.
- 协同方法恢复了具有β-1,3和β-1,6链接的多种类型的寡糖,证实了酵母β-葡萄糖的分支结构.
结论:
- 一个综合的物理化学和生物催化战略有效地将BSYβ-葡萄糖转化为有价值的寡糖.
- 这种方法为提升酒厂废物的价值提供了一个可持续的途径.
- 这些发现支持在饮料行业实施循环经济原则.
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