生物制造的细菌纤维素微载体用于培养肉类应用
Yue Li1, Ning Xiang2, Chenchen Shi1
1State Key Laboratory (SKL) of Biobased Transportation Fuel Technology, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Institute of Zhejiang University-Quzhou, 99 Zheda Road, Quzhou, Zhejiang Province 324000, China.
Food research international (Ottawa, Ont.)
|November 1, 2025
概括
研究人员为培养肉类开发了细菌纤维素 (BC) 微载体. 这些生物制造的微载体支持细胞生长和分化,从而改善培养肉类产品,增强质地和感官特性.
科学领域:
- 生物材料工程 生物材料工程
- 细胞农业 细胞农业
- 食品科学 食品科学 食品科学
背景情况:
- 培养肉的商业化需要可扩展和高效的微载体系统.
- 细菌纤维素 (BC) 为组织工程提供了一个有前途的生物相容性支架.
研究的目的:
- 为培养肉类生产开发生物制造的细菌纤维素 (BC) 微载体.
- 评估这些微载体在支持骨肌细胞 (CSM) 增殖和分化的有效性.
- 评估微载体培养肉对感官和纹理特性的影响.
主要方法:
- 在甲酸盐微球中封装Acetobacter xylinum,以创建结构化的BC微球.
- 用素修改BC微载体的表面,以增强细胞粘附.
- 在微载体上共培养骨肌细胞 (CSM) 和DF-1纤维细胞.
- 使用差异化微组织培养肉丸的构造和感官评估.
主要成果:
- 基托桑修饰的BC微载体显著改善了CSM细胞粘附,增殖和DF-1纤维细胞的脂肪转基因转分.
- 共同培养通过ECM沉积和分泌因子增强了CSM细胞的增殖和分化.
- 结合脂肪微组织的培养肉丸表现出改善的感觉和纹理属性,与天然肉丸相比.
结论:
- 生物制造的BC微载体为培养肉的开发提供了一个可扩展的,非动物衍生的平台.
- 这种方法为实现成本效益和可持续的替代蛋白质生产提供了可行的途径.
- 开发的微载体成功地在培养肉制品中模仿了天然肉的特性.
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