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氏菌 kandleri YT103: 转录组分析和肠道屏障保护的使用

Xin-Rui Zhang¹, Shuang-Shuang Cui¹, Long-Fen Yin¹

¹College of Life Science, Sichuan Normal University, Chengdu 610066, Sichuan, China.

Food research international (Ottawa, Ont.)

|November 1, 2025

概括

韦塞拉 kandleri 通过特定的酶利用西-酸糖 (XOS),增强肠道健康. 这项研究揭示了XOS代谢途径及其对协生菌的潜力,以改善肠道健康和对抗病原体.

科学领域:

微生物学微生物学
分子生物学分子生物学
肠道微生物组研究研究

背景情况:

氧-寡糖化物 (XOS) 是一种具有菌株特异性微生物利用的益生菌.
韦塞拉菌种类. 显示出益生菌潜力,但W. kandleri中的XOS代谢没有特征.
缺乏W. kandleriXOS代谢的多omics数据.

研究的目的:

阐明W. kandleri中XOS利用的分子机制 103.3.
通过综合基因组学和转录组学来识别参与XOS代谢的基因和途径.
评估XOS对W. kandleri生长,发酵产品和功能性质的影响.

主要方法:

对W. kandleri进行综合基因组和转录基因组分析 103.
识别编码酶和载体的基因集群.
增长动力学,酸化,qRT-PCR,短链脂肪酸分析和Caco-2细胞模型测试.

主要成果:

确定了编码GH43酶 (β-基酶,α-L-基酶) 和MFS载体的两个基因群.
XOS (DP 4-6) 显著增强了 W. kandleri YT103 的生长动力学和酸化.
XOS发酵增加了短链脂肪酸 (酸),表现出抗菌活性,并改善了肠道屏障的完整性.

结论:

这项研究澄清了W. kandleri YT103.3.中的XOS代谢途径.
已识别的基因为理解XOS利用提供了目标.
W. kandleri YT103 显示了协生发展的潜力,以改善肠道健康.

关键词:

肠道屏障肠道屏障肠道屏障文字转录学 (Transcriptomics) 是一个学科.威塞拉子 (Weissella kandleri) 是一种植物.氧化 - 氧化 - 氧化 - 氧化 - 氧化

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