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Updated: Jan 12, 2026

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微RNA链比率的混乱促进了质母细胞瘤中temozolomide的耐药性

Fangfang Shen^1,2, Pengfei Wu^3,4,5,6,7, Zelian Li^8,9

¹Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, 230027, Anhui, China.

Cellular & molecular biology letters

|November 1, 2025

概括

微RNA (miRNA) 链选择的混乱,特别是miR-92b,在质母细胞瘤 (GBM) 中增强了temozolomide (TMZ) 耐药性. 针对这种选择过程可能为GBM治疗提供新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

微RNA (miRNA) 链选择失调与各种癌症有关.
在质母细胞瘤 (GBM) 铁莫索洛米德 (TMZ) 耐药性中miRNA链选择的机制尚不清楚.

研究的目的:

调查miRNA链选择在GBM中TMZ耐药性的作用.
阐明改变miRNA链选择影响GBM化学抵抗的分子机制.

主要方法:

在耐TMZ的GBM细胞中分析miR-92b链表达模式.
通过向HDAC9和FOXP3.3,研究miR-92b-3p和miR-92b-5p对COL7A1表达的影响.
检查TUT4介导尿基化在前-miR-92b链选择中的作用.
评估TUT4抑制剂 (心中葡萄糖水合物-ATG-H) 在恢复TMZ敏感性方面的疗效.

主要成果:

较高的miR-92b-3p与miR-92b-5p比率与GBM中的TMZ抵抗有显著的相关性.
miR-92b-3p和miR-92b-5p调节的H3K27ac在COL7A1促进剂中的丰富,增加了COL7A1的表达和原沉积.
通过TUT4介导的尿化促进了前-miR-92b的3'链 (3p) 选择.
ATG-H治疗逆转了miR-92b链选择失调,并恢复了耐性GBM细胞中的TMZ敏感性.

结论:

在TUT4驱动的miR-92b-3p/-5p比率的升高促进了COL7A1转录,导致原沉积和增加了GBM中的TMZ抵抗.
准miR-92b链选择是一个潜在的治疗策略,以使GBM对TMZ敏感.

关键词:

质母细胞瘤 (glioblastoma) 是一个线程选择线程选择在Temozolomide的耐药性方面.尿化尿化这是一个微型RNA.

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MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...