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Updated: Jan 6, 2026

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
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Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

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通过一种简单的生物表达方法进行侧链选择性标签可以提高分辨率,并简化H检测蛋白固态NMR中的光谱

Yoshiki Shigemitsu1,2, Yuki Miyazaki2, Hibiki Terami2

  • 1School of Life Science and Technology, Institute of Science Tokyo, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.

Journal of biomolecular NMR
|November 1, 2025
PubMed
概括

我们开发了一种使用大肠杆菌的新型蛋白质标记方法,用于改进固态NMR (SSNMR) 分析. 这种技术增强了光谱分辨率,并简化了蛋白质结构确定中的信号分配.

关键词:
同位素标签的标记中等开关的交换机.选择性的质子化.固态NMR是一种固态NMR.

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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels11:19

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