ALYREF表达与上调的YBX1和HIF1α相关,有助于胃癌细胞迁移
Yujie Yuan1, Jiaqi Qiu1, Wenqing Tang2
1Department of Medical Genetics and Developmental Biology, School of Medicine, The Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, Nanjing 210009, China.
Tissue & cell
|November 1, 2025
概括
Y盒结合蛋白1 (YBX1) 和ALY环氧化酶类蛋白 (ALYREF) 在胃癌 (GC) 中被上调,并促进细胞迁移. 它们与HIF1α的相互作用可能会推动GC的进展,提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在瘤发生过程中,RNA 5-甲基细胞因 (m5C) 修改至关重要.
- 像YBX1和ALYREF这样的蛋白质充当m5C"读者",调节RNA代谢.
- 它们在胃癌 (GC) 发病过程中的特定作用需要阐明.
研究的目的:
- 为了研究YBX1和ALYREF在GC中的表达和功能.
- 探索它们在GC进展中的作用背后的分子机制.
- 确定潜在的诊断生物标志物和GC的治疗点.
主要方法:
- 对GC组织中的YBX1和ALYREF表达的分析与正常对照对比.
- 基因和基因组的京都百科全书 (KEGG) 和基因本体学 (GO) 对YBX1/ALYREF相互作用基因的丰富分析.
- 在体外细胞迁移测定.
- 与HIF1α和CagA状态的相关性分析.
- 定量PCR (qPCR) 和转录因子结合部位的预测.
主要成果:
- 在GC组织中,YBX1和ALYREF的表达显著更高.
- 与YBX1/ALYREF相关的丰富基因参与细胞迁移.
- YBX1和ALYREF都在体外增强了GC细胞迁移.
- ALYREF的表达与HIF1α和CagA阳性状态相关,表明与Helicobacter pylori感染的联系.
- YBX1可能调节ALYREF表达,而YBX1/ALYREF可能共同调节HIF1α.
结论:
- 通过增强细胞迁移,YBX1和ALYREF促进了GC的进展.
- YBX1/ALYREF-HIF1α轴与GC恶性瘤有关.
- 这些发现为GC诊断和表观遗传药物开发提供了新的见解.
更多相关视频
09:55All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
Published on: December 20, 2021
3.3K
11:32Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
7.4K
相关概念视频
02:38Mitogens and the Cell Cycle
7.7K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.7K
01:19Cell Migration
6.4K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.4K
01:09Cell Migration
18.5K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.5K
01:21Cell Polarization by Rho Proteins
3.5K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.5K
01:32Cytoskeletal Coordination in Cell Migration
5.4K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.4K
02:23Abnormal Proliferation
5.1K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K