在分散型微固态相提取黑茶样本中的真菌毒素中使用3D共价有机框架的应用,同时使用基于维生素的离子液作为化溶剂的溶剂
Fariba Khodadaeian1, Mohammad Reza Afshar Mogaddam2, Ali Mohebbi3
1Department of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran; Pharmaceutical Analysis Research Center, Pharmaceutical Sciences Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Journal of chromatography. A
|November 1, 2025
概括
一种新的共价有机框架 (COF) 方法有效地利用离子液体溶解剂从黑茶中提取亚毒素和毒素A. 这种技术提供了灵敏的检测,并成功地应用于真正的茶样.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 非洲毒素 (AFs) 和 ochratoxin A 是重要的真菌毒素,污染了黑茶等食品.
- 准确而敏感的检测方法对于食品安全监测至关重要.
研究的目的:
- 开发一种基于共价有机框架 (COF) 的新型分散型微固态相提取 (DμSPE) 方法,用于黑茶中的 AF 和 ochratoxin A.
- 使用液体染色学-并联质谱法 (LC-MS/MS) 来量化提取的毒素.
主要方法:
- 使用合成的三维 (3D) -COF作为吸附剂用于真菌毒素提取.
- 使用一种基于维生素的离子液体 (IL),胆尼古丁酸盐,作为一种无毒的化剂.
- 嵌入的乙烯基胺-n-propyl (PSA) 颗粒用于去除干扰化合物,如多.
主要成果:
- 实现了从0.011-0.031 ng/g的低检测极限 (LODs).
- 证明了0.103-250 ng/g的宽线性范围 (LRs),良好的相对标准偏差 (RSDs) ≤8.9%.
- 报告了高提取回收率 (ERs) 在72-80%之间,并证实了分析的茶样中含有亚黄素B1和绿素A的存在.
结论:
- 开发的基于COF的DμSPE方法对于黑茶中的真菌毒素分析是有效和敏感的.
- 使用离子液体溶解剂为传统有毒溶剂提供了更绿色的替代方案.
- 该方法的性能使其适用于常规食品安全分析.
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