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在真实样本和活细胞中检测HSO₃^-/SO₃^2-和粘度的多功能光探针

Yehao Yan¹, Kexin Zheng², Jianfeng Wang¹

¹School of Public Health, Jining Medical University, Jining, Shandong 272067, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|November 1, 2025

概括

一个新的光探测器JFL可以跟踪食物和细胞中的硫酸盐和粘度. 该工具为生理和食品安全监测提供了敏感和选择性的检测.

科学领域:

分析化学分析化学
生物化学生物化学
分子探头分子探头

背景情况:

异常的硫酸盐和粘度水平与生理过程有关.
硫酸盐是食品和活细胞中常见的可减少物种.
需要一个分子工具来跟踪硫酸盐和粘度.

研究的目的:

开发一种新的光探针,用于检测硫酸盐和粘度.
描述探测器的光学特性和传感能力.

主要方法:

合成了一个基于FRET/TICT的光探针 (JFL).
研究了探测器对硫酸盐和粘度的比度反应.
评估了检测极限,线性范围,灵敏度和选择性.

主要成果:

JFL 呈现深红色发射 (628 nm) 和大斯托克斯转移 (178 nm).
它通过绿色 (536 nm) 和红色 (628 nm) 排放的比度变化来监测硫酸盐.
JFL显示了低检测极限,高灵敏度和对硫酸盐 (0-100μM) 的选择性.
粘度检测是通过增加红色发射强度来实现的.
探测器在真实食物样本和活细胞中成功检测到硫酸盐和粘度.

结论:

JFL是一种有效的工具,用于比度硫酸盐检测和粘度传感.
该探测器在食品分析和生物成像方面有潜在的应用.

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