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Updated: Jan 12, 2026

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对于冷保存的细胞和组织样本的微尺度细胞表面蛋白质学.

John R Thorup1, Sarah A Johnston2, Moe Haines1

  • 1Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Molecular & cellular proteomics : MCP
|November 1, 2025
PubMed
概括

优化的表面蛋白质分析方法通过从低输入和冷保存样本进行强大的细胞表面蛋白质分析来改善诊断和治疗. 这些技术增强了用于患者分层的生物标志物发现.

关键词:
在N-glycoproteome中,它们具有N-glycoproteome的作用.细胞表面蛋白质组学细胞表面蛋白质组学质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质组学 蛋白质组学表面ome 表面ome 表面ome

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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