Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mitochondria01:37

Mitochondria

19.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
19.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.

HemaSphere·2026
Same author

From Sea to Cell: <i>Ascophyllum nodosum</i> and <i>Fucus vesiculosus</i> Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity-A Comprehensive Analysis Applying In Vitro and In Vivo Models.

Marine drugs·2026
Same author

Monitoring Nanoparticle Interaction with Murine Breast Cancer Cells Using Multimodal Fluorescence Lifetime Microscopy.

International journal of molecular sciences·2026
Same author

Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.

Journal of translational medicine·2026
Same author

Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.

Developmental cell·2025
Same author

Homeostatic Counter-Regulation Mediates Spermidine-Induced Triacylglyceride Reduction in Drosophila melanogaster-From Phenotype to Molecular Mechanism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

相关实验视频

Updated: Jan 12, 2026

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
09:18

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Published on: March 30, 2017

8.2K

细胞衰老 - - 在活细胞显微镜 (迷你检查) 中的一个重要因素.

Tina Karimian1, Christoph Cremer2, Julian Weghuber3

  • 1University of Applied Sciences Upper Austria, 4600, Wels, Austria.

Progress in biophysics and molecular biology
|November 1, 2025
PubMed
概括

细胞衰老会改变细胞结构和功能,影响活细胞显微镜的结果. 为了准确的实验设计和数据解释,研究人员必须考虑细胞通道数和衰老标记.

关键词:
光显微镜的光学显微镜.光显微镜的光学显微镜.活细胞是指活细胞.衰老是一种衰老.细胞通道号码 细胞通道号码

更多相关视频

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
10:41

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Published on: August 20, 2013

13.3K
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

6.4K

相关实验视频

Last Updated: Jan 12, 2026

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
09:18

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Published on: March 30, 2017

8.2K
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
10:41

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Published on: August 20, 2013

13.3K
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

6.4K

科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜技术 显微镜技术

背景情况:

  • 活细胞显微镜对于研究细胞对环境刺激的反应至关重要.
  • 细胞衰老导致细胞结构和功能发生显著变化,可能会扭曲显微镜数据.

研究的目的:

  • 审查细胞衰老如何影响亚细胞区和活细胞成像结果.
  • 专注于细胞衰老对先进显微镜和微操作技术的影响.

主要方法:

  • 文献综述专注于细胞衰老对显微镜的影响.
  • 对基因表达,核结构,新陈代谢和机械性质的变化进行分析.
  • 检查先进的技术:超分辨率,FLIM,VA-TIRFM和激光辅助的光孔.

主要成果:

  • 细胞衰老改变了关键的亚细胞区,影响了活细胞成像.
  • 衰老影响了先进显微镜和微操作的性能和结果.
  • 特定的变化会影响核结构,能量代谢和细胞膜机制.

结论:

  • 细胞衰老显著影响活细胞显微镜读数.
  • 实验设计和数据解释必须考虑到细胞通道数和衰老.
  • 了解衰老的影响对于准确的活细胞成像研究至关重要.