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Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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针对多发性骨髓瘤的先进多色流细胞计方法

Noa Ofir1, Ety Rozenberg1, Omri Sharabi1

  • 1The Shraga Segal Department for Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel.

Clinical lymphoma, myeloma & leukemia
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概括
此摘要是机器生成的。

一个新的14色流细胞计 (FACS) 协议有效检测多发性髓瘤 (MM) 骨髓中的克隆性. 这种方法有助于可视化复杂的血细胞异质性,以改善诊断和个性化治疗策略.

关键词:
克隆性 克隆性是指克隆性传真 (FACS) 是一个事实.异质性 异质性 异质性

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科学领域:

  • 血液学 血液学 血液学
  • 免疫学 免疫学 免疫学
  • 在瘤学瘤学.

背景情况:

  • 多发性骨髓瘤 (MM) 是骨髓的恶性血细胞疾病.
  • 尽管在治疗方面取得了进展,但MM由于复杂的细胞克隆性而仍然无法治愈.
  • 准确检测MM克隆性对于诊断和治疗至关重要.

研究的目的:

  • 提出一种新的14色流细胞计 (FACS) 协议,用于对MM克隆性进行可靠的检测和可视化.
  • 为了简化分析骨髓等离子体细胞的工作流程.

主要方法:

  • 为新鲜骨髓样本开发一个单管14色FACS协议.
  • 使用稳定,预混合的抗体尾酒,以提高可复制性.
  • 应用t分布式静态邻居嵌入 (t-SNE) 来可视化血细胞克隆性.

主要成果:

  • 14色FACS面板成功识别了CD38+CD138+血细胞及其克隆性.
  • 在诊断时,MM患者表现出独特的克隆模式和显著的异质性.
  • t-SNE提供了多参数血细胞异质性的统一二维可视化,揭示了患者在原发性和复发性疾病之间的相似性和差异.

结论:

  • 开发的FACS协议为MM诊断提供了一种强大而有效的方法.
  • 商用抗体和标准FACS设备与本协议兼容.
  • 多参数FACS数据的简洁可视化可以指导MM的治疗决策和个性化治疗策略.