Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ligand Binding Sites02:40

Ligand Binding Sites

8.5K
8.5K
Ligand Binding Sites02:40

Ligand Binding Sites

14.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
14.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

From generalization to precision: A large domain-specific pretrained model for specialized medical tasks.

Cell reports. Medicine·2026
Same author

Automated diagnosis of keratitis from low-quality slit-lamp images using an improved generative adversarial network.

NPJ digital medicine·2026
Same author

Pharmacological and Transcriptomic Exploration of β2-Adrenergic Receptor-Gα15 Signaling in THP-1-Derived Macrophages.

bioRxiv : the preprint server for biology·2026
Same author

Protease-activated receptor 1 as an endogenous model of peptidergic Gαq-Gα12-biased G protein signaling.

Frontiers in molecular biosciences·2026
Same author

De novo design of miniproteins targeting GPCRs.

Nature·2026
Same author

A Degron Decoy System Co-opts Pathological Seeding to Enable Clearance of Multimeric α-Synuclein.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Jan 6, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.9K

该APEXTAC系统用于基导近距离标记.

Min Ma1, YiYu Wang1, Johannes E Koeckenberger1

  • 1Department of Chemistry and Biochemistry, University of California San Diego, San Diego, CA, 92093, USA.

Chembiochem : a European journal of chemical biology
|November 2, 2025
PubMed
概括
此摘要是机器生成的。

新的APEXTAC系统可以在活细胞中无偏见地识别药物标. 它克服了BioTAC的局限性,为蛋白质平衡研究提供更快的标签和更高的准确性.

关键词:
在APEX2中,APEX2是APEX2的第二个版本.目标ID 目标ID 目标ID接近性标签的标签.

更多相关视频

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

13.2K

相关实验视频

Last Updated: Jan 6, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.9K
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

13.2K

科学领域:

  • 化学生物学 化学生物学
  • 分子生物学分子生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 公正的药物标识对于理解药物机制和非标效应至关重要.
  • 像BioTAC这样的现有方法有局限性,包括特定的化学成分和标签持续时间.
  • 在活细胞研究中,显然需要改进近距离标记工具.

研究的目的:

  • 开发和验证APEXTAC系统,一个新的小分子引导的近距离标签平台.
  • 为了将APEXTAC与BioTAC系统进行对比,用于药物标识.
  • 评估APEXTAC在研究蛋白质稳态和向蛋白质降解方面的实用性.

主要方法:

  • 使用APEX2过氧化酶的APEXTAC系统的开发.
  • 使用 (+) -JQ1进行目标识别,对比APEXTAC和BioTAC.
  • 对活细胞的标记效率和特异性的评估.

主要成果:

  • 在没有蛋白质酶抑制剂的情况下,APEXTAC通过它们的配体成功标记了E3酶.
  • 与BioTAC相比,APEXTAC系统证明了更快,可能更准确的目标识别.
  • APEXTAC在各种分子中对活细胞标识有效,包括蛋白质平衡元件.

结论:

  • APEXTAC 是一个有价值的补充工具,用于无偏见的活细胞药物标识.
  • 该系统克服了以前的近距离标签方法的关键局限性.
  • 在针对性蛋白质降解研究中,APEXTAC具有很大的应用潜力.