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通过全转录组测序建立一个ceRNA调节网络,用于西藏的繁殖性能

Fuzhou Liu¹, Yourong Ye¹, Yufan Gao¹

¹College of Animal Science, Xizang Agriculture and Animal Husbandry University, Linzhi, Xizang 860000, China; The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, , Linzhi, Xizang 860000, China; Key Laboratory of Tibetan Pig Genetic Improvement and Reproduction Engineering, Linzhi, Xizang 860000, China.

Animal reproduction science

|November 2, 2025

概括

西藏的卵产量较低,这是由于它们的生殖效率低于最佳. 这项研究确定了卵巢组织中的关键基因和调节网络,这些基因和调节网络可以解释这些差异,为保护和繁殖提供了洞察力.

科学领域:

动物遗传学动物遗传学
生殖生物学生殖生物学
禽畜科学禽畜科学禽畜科学

背景情况:

西藏 (TC) 是一个有价值的土著家禽资源,具有独特的蛋和装饰性特征.
低于最佳的生长和低的生殖效率限制了TC的更广泛的应用.
关于TC生殖功能的分子机制,特别是在卵巢组织中,尚未得到充分的研究.

研究的目的:

调查藏 (TC) 和罗马 (RC) 之间的生殖性能差异背后的分子机制.
构建与TC生殖效率相关的竞争性内源RNA (ceRNA) 调节网络.

主要方法:

在30天内,TC和RC之间的卵子生产率和激素水平 (卵泡刺激激素和孕激素) 的比较分析.
来自TC和RC的卵巢组织的全转录组测序 (RNA-seq).
基于差异表达基因 (mRNAs, lncRNAs, miRNAs) 的ceRNA调节网络的构建.

主要成果:

罗曼的产卵率和荷尔蒙水平明显高于西藏.
通过RNA-seq检测发现了基因表达的实质性差异,两组之间有2314个DEmRNA,532个DElncRNA和148个DEmiRNA.
一个ceRNA网络成功构建,突出了潜在的监管途径.

结论:

在ceRNA网络中识别的mRNAs,lncRNAs和miRNAs可能对调节藏式的毛囊发育和激素分泌至关重要.
这些发现为未来研究TC生殖表现的分子调节提供了基础的理解.
这项研究为藏族的潜在保护和选择性繁殖策略提供了见解.

关键词:

CeRNA 监管网络 CeRNA 的监管网络.卵巢卵巢卵巢生殖性表现的生殖性表现.西藏的肉,西藏的肉.整个转录组的转录组.

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