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对转录组进行比较分析,揭示了无皮大麦中盐耐受性的关键 lncRNA 和调节机制

Jakkrit Sreesaeng¹, Shi-Jun Li², Zhong-Hua Chen³

¹Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China; Expert Centre of Innovative Agriculture, Thailand Institute of Scientific and Technological Research, Pathum Thani, 12120, Thailand.

Plant physiology and biochemistry : PPB

|November 2, 2025

概括

这项研究在无皮大麦根中确定了新的长非编码RNA (lncRNA),揭示了关键的盐反应 lncRNA及其向基因. 这些发现为改善作物盐耐受性提供了洞察力.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
农作物科学农作物科学

背景情况:

盐应激严重影响全球作物生产.
长非编码RNAs (lncRNAs) 通过调节基因表达和信号通路,在植物应激反应中发挥关键作用.

研究的目的:

在盐耐受性 (X349) 和盐敏感性 (X66) 无皮大麦基因型的根部中识别和描述对盐敏感的 lncRNA.
阐明这些lncRNAs在盐分耐受性中的调节机制和功能作用.

主要方法:

在盐应激下对大麦根中的lncRNA进行比较转录组分析.
识别新型和差异表达的lncRNA及其向基因.
生物信息学分析包括cis-和trans-acting调节机制和功能丰富分析.

主要成果:

鉴定了7,648个新的lncRNAs,其中2,210个具有盐应激反应能力,与4,899个目标基因相关.
确定了139个lncRNA-mRNA对,其中123个是cis作用的,大多数目标基因 (4,776) 是通过转变作用机制调节的.
盐耐受性基因型中的差异表达的lncRNA与氨酸代谢,代谢,氨酸生物合成和氨基酸代谢有关,涉及诸如基因酶和氨酸氨基酶等基因.

结论:

对盐敏感的lncRNA及其向基因,特别是那些参与特定代谢途径并编码基因酶,氨酸氨基酶和壁相关受体激酶的基因,对于无皮大麦的盐耐受性至关重要.
这些发现突出了lncRNAs作为作物盐耐受性遗传改进的目标的潜力.
需要进一步的功能验证才能充分理解底层机制.

关键词:

基因酸酶的使用方法高通量RNA测序的高通量RNA测序黄 (Hordeum vulgare L.) 是一种常见的植物.盐的压力是盐的压力.与墙壁相关的受体激酶.在ncRNA中,我们可以看到

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