三种方法的比较,声称生物膜消毒的三种方法
I Beaugelin1, S Bulot1, L Pineau1
1Eurofins Biotech Germande, Aix en Provence, France.
The Journal of hospital infection
|November 2, 2025
概括
消毒剂对生物膜的有效性取决于生物膜的成熟度. 成熟的生物膜,长得更长,对消毒剂的敏感性较低,因此需要将它们纳入医疗保健机构的疗效测试中.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 生物技术是生物技术.
背景情况:
- 生物膜是医院感染的重要原因之一.
- 有效的消毒剂对于使生物膜内的细菌失活至关重要.
- 缺乏对生物膜的标准化消毒有效性测试.
研究的目的:
- 描述不同的Pseudomonas aeruginosa生物膜生长方法.
- 评估不同成熟度的生物膜对消毒剂的敏感性.
- 根据对生物膜的消毒剂疗效的标准化测试方案提供信息.
主要方法:
- 描述了三种Pseudomonas aeruginosa生物膜生长方法 (ISO 15883-5,ASTM E2562,Konrat等. ) 的情况.
- 量化蛋白质,总有机碳 (TOC) 和细菌度.
- 使用基于NF EN 14561的方法与酸 (PAA) 评估消毒剂的敏感性.
主要成果:
- 在ISO 15883-5生物膜中,蛋白质和细菌含量更高.
- 长时间生长的生物膜 (例如96小时) 显示消毒剂敏感性降低.
- 较老,更成熟的生物膜对消毒剂失活的敏感性较低.
结论:
- 生物膜成熟,受生长时间的影响,与降低消毒剂敏感性直接相关.
- 消毒功效测试应包括成熟的生物膜,以反映现实条件.
- 医疗器械上的消毒剂的标准化测试必须包括成熟的生物膜挑战.
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