部分化-安纳摩克斯过程中微生物基因多态的适应,以保持在盐度应激下脱性能和强度
Risen Yang1, Yanxiao Wei2, Jianhong Jiang3
1School of Hydraulic and Ocean Engineering, Changsha University of Science & Technology, Changsha 410114, China; Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha 410114, China.
Journal of environmental sciences (China)
|November 2, 2025
概括
部分化-亚纳摩克斯 (PNA) 过程通过丰富 osmoprotective 基因和激活代谢通路来适应高盐度. 这提高了无氧氨氧化细菌 (AnAOB) 的盐耐受性,确保了厨房废水中的有效去除.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 生物反应器工程学
背景情况:
- 厨房废水由于盐度高和氨度高,因此存在挑战.
- 部分化-安纳摩克斯 (PNA) 是一个有前途的去除的过程,但其在盐水条件下的有效性尚不清楚.
- 了解微生物适应机制对于优化高盐度环境中的PNA至关重要.
研究的目的:
- 为了研究在高盐度压力下PNA过程的适应策略.
- 使用元基因组分析阐明微生物群落的生理和分子反应.
- 评估一种新型空运内部循环隔离生物反应器 (AIPBR) 的性能,用于高盐度废水处理.
主要方法:
- 在不同盐度 (1-10 g/L) 和高负载下使用合成废水的AIPBR运行.
- 高通量元基因组测序用于分析微生物社区结构和功能基因的变化.
- 分析关键的代谢途径,包括 osmoprotectant 合成和能量生产 (TCA 循环).
- 评估除效率和系统稳定性的评估.
主要成果:
- 甲基因组分析显示,阿纳莫克斯细菌中的基因富含用于奥斯莫保护性化合物合成和TCA循环激活的基因.
- 无氧氨氧化细菌 (AnAOB) 显示了增强的盐耐受性,并保持了超过85%的总去除效率.
- 观察到与离子运输,应激反应和DNA修复相关的基因的显著丰富.
- 微生物群落的转移有利于耐盐物种,有助于整体系统的稳定性.
结论:
- 通过特定的微生物生理和分子机制,PNA过程可以有效地适应高盐度条件.
- 开发的AIPBR适合扩大PNA,以处理具有挑战性的高盐度废水.
- 这项研究为在盐度较高的环境中可持续地去除提供了一种有希望的方法.
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