相关实验视频

Updated: Jan 12, 2026

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

在Pseudomonas syringae pv.有效的基因组编辑. 在使用CRISPR/FnCas12a系统的行为体中

Zhenzhen Gou^1,2, Yue Wang¹, Chunyi Qin³

¹School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China.

Molecular horticulture

|November 2, 2025

概括

我们开发了一个CRISPR/FnCas12a基因编辑系统,用于Pseudomonas syringae pv. 行动 (Psa),一种果的病原体. 这个系统使得功能基因研究成为可能,并揭示了果-Psa病理系统中的效应器-标相互作用.

科学领域:

植物病理学植物病理学
微生物遗传学微生物遗传学
分子生物学分子生物学

背景情况:

基于CRISPR的基因编辑在植物病原体中未得到充分利用.
伪虫的注射器 pv. (Psa) 导致果的细菌性癌症 (BC).
Psa biovar 3 (Psa3) 是全球BC流行病的原因之一.

研究的目的:

建立一个用于Psa基因编辑的CRISPR/FnCas12a系统.
调查HopH1和HopZ5因子在Psa病毒性中所起的作用.
在果-Psa病理系统中探索效应器-宿主相互作用.

主要方法:

在pBBR1-MCS2向量中构建了CRISPR/FnCas12a系统,用于Psa.
设计了CRISPRRNAs (crRNAs) 来准hopH1和hopZ5基因.
使用PCR原料来选基因删除和载体存在.

主要成果:

在Psa中使用CRISPR/FnCas12a.成功建立了基因组编辑.
证明crRNA准位置影响基因删除效率.
双重突变的 ΔhopZ5ΔhopH1 在不同的果品种上表现出改变的毒性,这表明了效应者与宿主之间的相互作用.

结论:

克里斯普尔/FnCas12a系统是有效的基因编辑在Psa.
hopH1和hopZ5效应器在Psa毒性中起作用,并与果耐药基因相互作用.
该系统有助于病原体基因功能分析和效应器-标相互作用研究.

关键词:

细菌性癌症是一种细菌性癌症.效应器效应器效应器效应器果 (Kiwi) 是一种果.突变突变突变突变突变病毒性病毒性

更多相关视频

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

The Antiviral System of Bacteria and Archaea: CRISPR

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...