相关实验视频
Updated: Jan 12, 2026

06:08
Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
11.8K
不同的黑色素漂白方法对染色的影响
Xinpeng Liu1, Peng Li1, Wenjuan Jia1
1Department of Pathology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China.
Applied immunohistochemistry & molecular morphology : AIMM
|November 3, 2025
概括
黑色素漂白对于准确的病理诊断至关重要. 过氧化治疗是最温和的方法,保留组织染色和抗体表达,以获得可靠的诊断结果.
科学领域:
- 病理学 病理学 病理学
- 历史学 历史学 历史学
- 免疫组织化学 免疫组织化学
背景情况:
- 黑色素色素可以干扰组织样本中准确的病理诊断.
- 有效的黑色素漂白技术对于可靠的组织学和免疫组织化学染色至关重要.
- 以前的方法可能会损害抗原性和组织形态.
研究的目的:
- 评估不同黑色素漂白方法对血素-氨酸 (H&E) 染色的影响.
- 评估黑色素漂白对特定抗体免疫组织化学 (IHC) 染色的影响.
- 确定最佳的黑色素漂白协议,以保持组织完整性和抗原性.
主要方法:
- 三种黑色素漂白技术的比较: permanganate/oxalic acid,过氧化和双氧化.
- 对恶性黑色素瘤组织和对照组织 (T细胞淋巴瘤,B细胞淋巴瘤,神经纤维瘤,结肠) 应用漂白方法.
- 对CD3,MUM1,CD20和S-100进行H&E染色质量和IHC染色的评估.
主要成果:
- permanganate/oxalic 酸治疗导致 CD3 和 MUM1 抗体的负表达.
- 过氧化治疗维持了CD3,MUM1,CD20和S-100抗体的正常表达.
- 在37°C下24小时使用10%的过氧化对H&E和IHC染色的影响最小,被证明是最温和有效的方法.
结论:
- 10%的过氧化是保持H&E和IHC染色的诊断精度的最佳黑色素漂白剂.
- 这种方法适用于广泛的组织和抗体,最大限度地减少诊断人工物.
- 有效的黑色素漂白提高了在有色素的组织中对组织病理学诊断的可靠性.
相关概念视频
Fixation and Sectioning
7.3K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
7.3K
Pigmentation
4.1K
The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
4.1K
Differential Staining Technique
2.0K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.0K
Simple Staining Technique
3.0K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
3.0K
Changes in Skin Color: Clinical Perspectives
3.3K
The first thing a clinician sees is the skin, so the examination of the skin should be part of any thorough physical examination. Most skin disorders are relatively benign, but a few, including melanomas, can be fatal if untreated. A couple of the more noticeable disorders, albinism and vitiligo, affect the appearance of the skin and its accessory organs.
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
3.3K
Special Staining Techniques
1.1K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.1K

