回头看向前:青酸在寄生原生动物的TEM中
Sharmila Ortiz1, Wanderley de Souza1, Marlene Benchimol2,3
1Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Pesquisa em Medicina de Precisão, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
概括
酸改善了传输电子显微镜 (TEM) 中的对比度,用于像Giardia intestinalis这样的原生动物. 这种安全且具有成本效益的方法增强了细胞结构的可视化,简化了TEM协议.
科学领域:
- 细胞生物学 细胞生物学
- 显微镜技术 显微镜技术
背景情况:
- 传输电子显微镜 (TEM) 需要优化固定和对比增强用于超结构分析.
- 像Giardia intestinalis和Trichomonas vaginalis这样的原生动物样本,由于其复杂的形态,对TEM提出了挑战.
研究的目的:
- 评估酸作为Giardia intestinalis和Trichomonas vaginalis的TEM mordant和对比剂的作用.
- 评估酸在改善超结构可视化和简化TEM协议方面的有效性.
主要方法:
- 酸被用作主要的固定杀伤剂,与氧化 (OsO4) 结合后固定.
- 酸也被测试为一个后染色剂,取代酸.
- 用传输电子显微镜分析了样品.
主要成果:
- 酸显著增强了等离子体膜,细胞器和细胞骨元素的区块内对比度.
- 它改善了基底体,轴膜和过渡区结构的可视化.
- 酸允许简化协议,包括可能省略酸和取代酸,而不会影响图像质量.
结论:
- 酸是一种安全,经济有效和高效的替代传统TEM对比剂.
- 它改进了用于研究原生动物细胞生物学和超结构的TEM协议,特别是在生物安全限制下.
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