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SbWRKY50通过调节侧向根形成来促进的耐旱性

Wei Chen¹, Zongkuan Wang², Chengzhuo Tao¹

¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.

Plant, cell & environment

|November 3, 2025

概括

一种新的 WRKY 转录因子,SbWRKY50,对的横向根部发育至关重要. 它调节乙烯生物合成和表观遗传沉默,增强干旱耐受性和作物产量.

科学领域:

植物生物学植物生物学
遗传学是一个遗传学.
农业学是一种农业学.

背景情况:

侧向根 (LR) 形成对植物结构和营养吸收至关重要.
乙烯在LR发育中起着关键的抑制作用,但核心调控转录因子 (TFs) 基本上是未知的.
了解LR法规对于提高作物产量和耐压能力至关重要.

研究的目的:

为了确定调节乙烯生物合成和Sorghum bicolor侧根发育的核心TFs.
阐明SbWRKY50控制侧向根形成的分子机制.

主要方法:

在 Sorghum bicolor 中进行基因表达分析.
在CRISPR/Cas9基因编辑中创建SbWRKY50突变.
对WRKY TF与目标基因的相互作用和表观遗传修饰的分析.

主要成果:

SbWRKY50积极调节横向根部发育;过度表达增加了LR的数量和长度,而突变者显示LR形成减少.
SbWRKY50直接抑制1-氨基cyclopropane-1-碳酸盐合成酶 (ACS) 基因,这是乙烯生物合成的关键.
SbWRKY50与SbBMI1A相互作用,以招募PRC1复合体,导致H2A无处不在和SbACS基因的表观遗传沉默.

结论:

SbWRKY50是一种关键的积极调节器,对的横向根部发育.
乙烯生物合成的SbWRKY50介导的表观遗传调节增强了横向根的形成.
SbWRKY50有助于改善农学特征,包括干旱耐受性和的产量潜力.

关键词:

这就是SbWRKY50的意义.Sorghum 双色球红杉双色球耐旱耐受性耐旱耐受性乙烯合成的方法

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