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血管性先于牙真菌干细胞中的神经性分化

R Tsuboi¹, Z Zhang¹, K Warner¹

¹Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.

Journal of dental research

|November 3, 2025

概括

牙髓干细胞 (DPSC) 是异质的,可以分化为各种细胞类型. 活体研究显示,DPSC首先形成血管,然后神经结构,受根运河环境的影响.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
口腔生物学口腔生物学

背景情况:

牙纸干细胞 (DPSCs) 是多能,神经衍生的细胞.
在体内了解人类DPSC分化机制至关重要,但仍然不清楚.

研究的目的:

阐明人类DPSCs的体内分化机制.
为了研究DPSC移植后的分化事件的序列.

主要方法:

诱导DPSCs的单细胞RNA测序用于体外分化 (血管性,口腔性,神经性).
将GFP标记的DPSC移植到免疫缺陷小鼠体内,然后在7日和21日进行流细胞计和单细胞RNA测序.
在体内对DPSC分化的时间分析.

主要成果:

基线DPSC表现出显著的异质性,有8个不同的集群.
在体外分化诱导了独特的基因表达特征.
在体内,干细胞标记物下调,内皮细胞标记物在7天后上调,神经元标记物在21天后上调.
移植后2周形成功能性血管,5周形成神经结构.

结论:

DPSCs是异质干细胞,具有不同的祖先种群.
在体内微环境,特别是人类的根通道,驱动着DPSCs的顺序血管和神经分化.

关键词:

血管新生是因为血管新生.神经科学/神经生物学牙的生长过程 (odontogenesis)纸生物学纸生物学一个单细胞RNA-seqq.血管生物学血管生物学

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