3D生物打印酶-细菌共生体,用于从纤维素生物转化生产乳酸
Ke-Wan Li1, Meng-Jie Luo1, Yixuan Wang2
1State Key Laboratory of Advanced Environmental Technology, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.
Environmental science & technology
|November 3, 2025
概括
这项研究开发了3D生物打印的酶-细菌共生体,用于从纤维素有效生产乳酸. 这种工程生物材料平台增强了废物的价值转化,并支持循环生物经济原则.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 材料科学 材料科学 材料科学
背景情况:
- 废弃纤维素生物质转换的传统微生物共同培养面临微生物竞争和空间组织不良等挑战,限制了效率和稳定性.
- 废弃物生物质的有效生物转化为高价值化学品对于可持续的工业过程和循环生物经济至关重要.
研究的目的:
- 通过3D生物打印来设计新的酶-细菌共生体,以从纤维素中增强乳酸生产.
- 开发一种生物相容的生物墨水,用于精确地安排酶和细菌的空间.
- 优化共生体的几何配置,以提高生物转化效率.
主要方法:
- 开发一种可生物相容的双网络功能性生物墨水,具有优化3D生物打印的rheological特性.
- 制造3D生物打印的酶-细菌共生体,具有多层结构 (内部细胞酶,外部细菌).
- 优化酶负载和纤维素度,以生产乳酸.
- 使用反应-扩散模拟来分析中间体和产品的空间分布.
主要成果:
- 使用3D生物打印共生体与17.5g/L纤维素在35U/mL的最佳酶负荷时,达到6.55±0.34g/L的最大乳酸产量.
- 通过优化3D生物打印结构中的空间布局,证明了乳酸生产效率的提高.
- 反应-扩散模拟提供了对共生体内葡萄糖和乳酸的空间动态的洞察.
结论:
- 通过对酶-细菌共生体的3D生物打印,为工程化生物材料建立了一个新的设计范式.
- 开发的平台为各种废物转化为产品的可扩展解决方案提供了可扩展的解决方案.
- 这项工作提供了实施循环生物经济原则的实际途径,通过利用废弃的纤维素生物质.
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