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非营养甜味剂对细菌定数感应和表型表达的影响:对肠道微生物组动态的影响

Mindani Watawana^1,2,3, Emilia Maria Franca Lima⁴, Beatriz Ximena Valencia Quecan⁴

¹Department of Biological Sciences, Faculty of Science and Engineering, University of Limerick, Limerick, Ireland.

Gut microbiome (Cambridge, England)

|November 3, 2025

概括

非营养甜味剂 (NNSs),如塔加和粉,可以抑制细菌的交流 (定数感应). 其他NNS也会影响微生物基因表达,这表明肠道微生物群的作用有所不同.

科学领域:

微生物学微生物学
生物化学生物化学
计算生物学计算生物学

背景情况:

非营养甜味剂 (NNSs) 是广泛使用的糖替代品,用于减少卡路里.
没有完全理解NNS对人类肠道微生物群的长期影响.
定数感应 (QS) 是一种影响微生物群落的关键细菌通信系统.

研究的目的:

调查四种NNSs (塔加,,Rebaudioside-A,糖) 对QS调节的表型和基因表达的影响.
探索这些NNSs对肠道微生物群的潜在影响.
通过分子对接来分析NNS与QS调节蛋白的结合亲和力.

主要方法:

利用QS生物传感器模型细菌, *Chromobacterium violaceum* 和 *Pseudomonas aeruginosa*,来评估NNS的影响.
测量了QS调节的表型 (例如,紫素的产生,蜂群的运动性) 和基因表达 (*lasI*).
进行了分子对接模拟,以评估NNS与QS受体的结合亲和力.

主要成果:

塔加和素以剂量依赖的方式显著抑制了QS表型,也降低了P. aeruginosa lasI*基因表达.
雷巴奥化物-A和糖显示出较少突出的QS抑制,但也减少了*lasI*表达.
分子对接显示,与和塔加相比,对撒卡林和Rebaudioside-A的结合亲和力更高.

结论:

不同的NNS对细菌QS系统产生不同的影响,影响微生物的交流.
观察到的效应是特定于物种的,并且依赖于剂量.
这些发现凸显了NNS消费对肠道微生物组组成和功能的潜在影响.

关键词:

这是一种Chromobacterium violaceum的细菌.伪omonas空气菌伪omonas空气菌雷巴乌迪oside-A 的使用.氨酸氨酸是一种氨酸.糖是一种糖.塔加托斯 (Tagatose) 是一个古老的语言.

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