在膀性口腔炎病毒感染期间,对宿主和病毒衍生的循环RNA进行全面分析
Shanshan Miao1, Zesen Mai1, Lu Zhu2
1Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Frontiers in cellular and infection microbiology
|November 3, 2025
概括
循环RNAs (circRNAs) 在膀性口腔炎病毒 (VSV) 感染中发挥作用. 主体和病毒cirRNA都受到VSV的上调,影响病毒复制和感染过程.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 循环RNA (circRNA) 是一种新的非编码RNA类,在病毒感染中获得了关注.
- 膀性口腔炎病毒 (VSV) 是一种负感RNA病毒,在有限的治疗条件下对畜牧业构成威胁.
- 系统性circRNA在VSV感染期间的概况以前没有被探索.
研究的目的:
- 在VSV感染的细胞中全面分析circRNA配置文件.
- 研究宿主和病毒circRNAs在VSV感染中的作用.
- 发现涉及circRNAs的新病毒与宿主相互作用.
主要方法:
- 在VSV感染的Vero细胞中进行circRNA分析的高通量测序.
- 对circRNAs的差异表达分析和趋势聚类.
- 功能性注释和circRNA特征的表征.
主要成果:
- 确定了65,645个宿主circRNAs (1,682个差异表达) 和120个病毒circRNAs.
- VSV感染上调了特定宿主和促进病毒复制的病毒circRNAs.
- 功能性注释将circRNA集群1与前病毒途径联系起来.
结论:
- VSV感染诱导了宿主细胞和病毒circRNAs.
- 这些circRNAs显著影响VSV感染和复制.
- 这项研究揭示了病毒与宿主相互作用的新层,其中包括用于VSV控制策略的circRNAs.
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