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Updated: Aug 12, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

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在多种不同的农业化学品中,表型定转录学揭示了斑马鱼的保存途径和独特的基因表达特征

Lindsey St Mary1, Ryan McClure2, Lisa Truong1

  • 1Sinnhuber Aquatic Research Laboratory, Environmental and Molecular Toxicology Department, College of Agricultural Sciences, Oregon State University, Corvallis, OR, United States.

Frontiers in toxicology
|November 3, 2025
PubMed
概括

这项研究使用斑马鱼来比较各种农业化学物质,揭示了共同的生物途径,如神经发育和细胞骨组织,尽管有不同的形态效应. 这为了解农业化学毒性和结构-活性关系提供了一个框架.

关键词:
农业化学品是一种农业化学品.碳硫二氧化碳的使用方法甲是一种甲.它们的作用模式.转录基因分析的概况斑马鱼是一种斑马鱼.

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Transgenic Organisms00:53

Transgenic Organisms

Overview
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From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
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DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
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What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...