一种新的非向分子检测技术,用于在非常早产婴儿的便微生物群的快速分析:优化,性别水平比较和应用
R R de Kroon1,2,3, A J van Wesemael1,2,3, A H van Kaam2,4
1Department of Pediatric Gastroenterology, Emma Children's Hospital, Amsterdam UMC, Amsterdam, the Netherlands.
概括
快速的IS-pro微生物群测定准确地描述了早产婴儿的肠道细菌,与16S测序具有很高的一致性. 这种快速,床边适用的工具有可能用于新生儿重症监护室 (NICU) 的临床实践.
科学领域:
- 微生物学 微生物学
- 新生儿医学 新生儿医学
- 生物信息学是一种生物信息学.
背景情况:
- 肠道微生物群的分析对新生儿重症监护室 (NICU) 护理至关重要.
- 目前的方法如16S rRNA和元基因组测序缺乏床边适用性.
- IS-pro测定在5小时内提供了快速的物种级丰度数据.
研究的目的:
- 优化IS-pro微生物群对早产婴儿的测定.
- 将IS-pro的发现与16S测序进行基因级别的比较.
- 将优化的IS-pro试验应用于早产婴儿队列.
主要方法:
- 使用纳米孔测序进行数据库扩展的IS-pro测试优化.
- 在41个便样本上对IS-pro和16S测序进行比较分析.
- 将优化的IS-pro试验应用于10个NICU的479名早产婴儿的1445个便样本.
主要成果:
- 经过优化后,平均相对丰度为82.5%被成功注释.
- 在IS-pro和16S测序之间具有很高的一致性 (皮尔森的r=0.77).
- 确定了占主导地位的属:葡萄球菌,Klebsiella,Enterococcus,Escherichia-Shigella和链球菌.
结论:
- IS-pro测定是一种快速有效的工具,用于检测和量化早产婴儿便样本中的细菌种群.
- IS-pro的结果与16S测序具有很高的一致性,证明了它的可靠性.
- 该试验的速度和准确性表明,在新生儿重症监护室有很大的临床应用潜力.
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