Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MicroRNAs01:22

MicroRNAs

23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
MicroRNAs01:22

MicroRNAs

3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes.

Nucleic acids research·2026
Same author

A long-distance signaling loop promotes soybean nodulation and productivity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Stage-Specific RNA Turnover Drives Small RNA Dynamics in <i>Arabidopsis</i>-<i>Colletotrichum</i> Interactions.

Plant direct·2026
Same author

The annotated blueprint: integrated functional genomic resources for a model tetraploid wheat Triticum turgidum cv Kronos.

The New phytologist·2026
Same author

Long-distance transport of siRNAs with functional roles in pollen development.

Nature plants·2026
Same author

De novo and inherited dominant variants in U4 and U6 snRNA genes cause retinitis pigmentosa.

Nature genetics·2026

相关实验视频

Updated: Jan 12, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.9K

miRScore:一个快速而精确的微RNA验证工具.

Allison Vanek1,2, Sam Griffiths-Jones3, Blake C Meyers4

  • 1Bioinformatics and Genomics Ph.D. Program, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America.

PLoS computational biology
|November 3, 2025
PubMed
概括

miRScore是一个新的工具,使用小RNA测序数据验证微RNA (miRNA) 注释. 它提高了新miRNA提交到数据库的准确性,提高了miRNA注释的整体质量.

更多相关视频

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.8K
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

6.5K

相关实验视频

Last Updated: Jan 12, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.9K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.8K
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

6.5K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 微RNAs (miRNAs) 是真核生物中至关重要的基因调节者.
  • 小RNA测序 (sRNA-seq) 是发现和注释miRNAs的关键.
  • 目前的方法往往导致错误阳性miRNA注释,需要更好的验证.

研究的目的:

  • 引入miRScore,这是一个独立的工具,用于验证新的miRNA注释.
  • 为从sRNA-seq数据中评估miRNA候选者的可靠性提供一种可靠的方法.

主要方法:

  • miRScore集成了基于sRNA-seq数据的结构和表达分析.
  • 它为评估miRNA注释提供了详细的指标和可视化.
  • 该工具旨在实现快速有效的验证.

主要成果:

  • miRScore提供了新的miRNA注释的可靠验证.
  • 它提高了用户对评估miRNA注释质量的信心.
  • 该工具有助于提高miRNA提交到数据库的准确性.

结论:

  • miRScore是一个有效和独立的工具,用于验证miRNA注释.
  • 它有可能显著提高miRNA数据库的质量.
  • miRScore还可以作为重新评估现有的miRNA注释的资源.