暴露在寒冷环境下会触发不同的RNA编辑,替代拼接和基因表达模式,在寒冷养的气球鱼 (Takifugu obscurus) 中
Shuang Han1, Jie Wang1, Yuhao Luo1
1International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, China; College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Lingang New City, Shanghai, 201306, China.
Fish & shellfish immunology
|November 3, 2025
概括
一种耐寒的气泡鱼菌株显示了能量代谢的增强,并在寒冷压力下减少了炎症. 这突出了关键的基因和途径,这些基因和途径对于改善鱼类耐寒性和水产养殖潜力至关重要.
科学领域:
- 水生生物学 水生生物学
- 鱼类生理学 鱼类生理学
- 基因组学就是基因组学.
背景情况:
- 低温挑战鱼类生理,影响生长,免疫力和生存,特别是在寒冷耐受性有限的物种中.
- 人工养成功地开发出一种耐寒的Takifugu obscurus (气泡鱼) 菌株,能够在极低的温度 (5°C) 中生存.
- 这种耐寒的菌株提供了一个独特的模型,用于研究远鱼的耐寒性背后的分子机制.
研究的目的:
- 为了研究气泡鱼对寒冷压力的生理和转录基因反应.
- 为了比较耐寒 (CT) 菌株和Takifugu obscurus的野生型 (WT) 菌株之间的寒冷应激机制.
- 通过综合分析,确定涉及寒冷耐受性的关键基因和途径.
主要方法:
- 在寒冷条件下对CT和WT气球鱼的状组织进行组织学分析.
- 使用权重基因共同表达网络 (WGCNA) 进行转录基因分析,以识别关键基因模块.
- 将WGCNA的结果与RNA替代拼接 (AS) 和RNA编辑的分析进行整合.
主要成果:
- 与WT在冷应力下相比,CT气球鱼表现出较少的病变和更好的结构完整性.
- WGCNA确定了由胰岛素,PPAR和mTOR信号调节的脂肪酸和葡萄糖代谢途径,对于CT气球鱼的寒冷耐受性至关重要.
- WT气球鱼显示炎症和亡通路的增加,而这两种菌株在寒冷压力下都参与了基切割修复和结合体通路.
- 富含MAPK和PPAR等途径的RNA替代拼接和编辑基因,随着温度的降低而增加.
- 确定了关键的冷适应基因 (疼痛,arsd,nectin4,polr3g,prr5),这些基因参与了氧化还原,免疫和能量调节.
结论:
- 在Takifugu obscurus中,耐寒性与强大的能量代谢和受控的炎症反应有关.
- RNA的替代拼接和编辑在鱼类的寒冷应激反应中起着重要作用.
- 鉴定的基因和通路为旨在增强水产养殖物种耐寒性的遗传育种计划提供了目标.
相关概念视频
Alternative RNA Splicing
24.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.6K
Alternative RNA Splicing
4.8K
4.8K
RNA Splicing
60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
RNA Editing
9.8K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.8K
Responses to Heat and Cold Stress
14.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.6K
Background and Environment Affect Phenotype
7.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.4K


