概括
我们开发了一种结合双视野和偏振成像的新型显微镜,用于实时活细胞分析. 这种极化分辨率的数字全息显微镜 (PDHM) 可以捕捉到详细的细胞结构和无标签的异质性.
科学领域:
- 生物医学光学 生物医学光学
- 显微镜的使用方法
- 细胞生物学 细胞生物学
背景情况:
- 传统显微镜通常需要标签或缺乏详细的极化信息来对活细胞进行成像.
- 同时获得多个区域和两极化状态是具有挑战性的.
研究的目的:
- 推出一种新的单射极化分辨率数字全息显微镜 (PDHM).
- 为了实现实时,无标签的活细胞成像,同时提供双视场 (DFoV) 和偏振信息.
主要方法:
- 使用萨格纳克干扰仪设置,将参考光束分割为直角偏振的组件,具有不同的空间载波频率.
- 对象路径中的光束分割器启用了角度复合,同时将两个样本区域捕获到一个传感器上.
- 独立重建两极分化解决的复杂场和空间信息在单个获取中实现了.
主要成果:
- 该PDHM系统成功地在实时中重建了活体MCF-7乳腺癌细胞中的相异性和内部细胞特征.
- 该系统能够同时捕获两个不同的样本区域,并提供两极化信息.
- 无标签,广场和偏振敏感成像在一个紧的格式被证明.
结论:
- 新的PDHM为先进的生物医学成像和动态细胞分析提供了强大的工具.
- 这种技术克服了传统方法的局限性,提供了丰富的,无标签的空间极化信息.
- 该系统的实时功能对于研究动态细胞过程至关重要.
相关概念视频
01:28Three-Dimensional Microscopy in Microbiology
755
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
755
01:26Phase Contrast and Differential Interference Contrast Microscopy
12.0K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
12.0K