在Salmonella enterica亚种中因干燥应激引起的转录组变化. 进入服务器阿戈纳阿戈纳
Sultana Solaiman1, Ellie Meeks1, Ian Hines2
1Genomics Development and Applications Branch, Division of Food Safety Genomics, Office of Applied Microbiology and Technology, Office of Laboratory Operations and Applied Science, Human Foods Program, U.S. Food and Drug Administration, College Park, MD 20740, United States.
沙门氏菌 (Salmonella Agona) 通过减少新陈代谢和复制,在低水分的食物中生存. 这种病原体.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 基因组学就是基因组学.
背景情况:
- 与谷物产品相关的沙门氏菌Agona (S. Agona) 爆发突出了其在低水分食品 (LMF) 中的持久性.
- 以前的全基因组测序表明,S. Agona在引起疫情之前可以在LMF行业长时间存在.
研究的目的:
- 阐明S. Agona在LMF中的生存背后的分子机制.
- 调查干燥应激对S. Agona基因表达和表型的影响.
主要方法:
- RNA测序 (RNA-seq) 用于分析在干燥应激下基因表达变化.
- 差异表达基因 (DEGs) 的功能分析.
- 基因淘汰实验,以评估突变生物在干燥压力下的生存率.
主要成果:
- 在干燥应激下,在S. Agona中发现了1114个差异表达基因 (DEG),其中646个下调和468个上调.
- 下调的基因主要参与代谢途径和翻译,表明生长减少.
- 包括kdp,ccm和tisB在内的上调基因与分别与生长调节,减少氧气消耗和持续形成有关.
- 与野生类型相比,具有被淘汰的操作子/基因的突变体在生存中显示出1-2日志的减少.
结论:
- S. Agona 采用节能,降低新陈代谢和减少复制的策略,在低湿度的食物环境中生存.
- 特定的上调基因 (kdp,ccm,tisB) 在S. Agona的干燥耐受性中起着至关重要的作用.
- 了解这些生存机制对于防止未来的食物传播疫情至关重要.
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