乳制品和植物乳制品替代品中的细菌和真菌群体组成使用高吞吐量测序
Dimitrios A Anagnostopoulos1, Evangelia A Karamani1, Stavroula Letsiou1
1Laboratory of Marketing and Technology of Aquatic Products and Foods, Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou street, 38446 Volos, Greece.
Food research international (Ottawa, Ont.)
|November 4, 2025
概括
基于植物的替代乳制品比乳制品具有更高的微生物多样性,存在有益和潜在有害的微生物. 有针对性的初始培养对于确保植物性发酵食品的安全性和质量至关重要.
科学领域:
- 食品微生物学 食品微生物学
- 发酵科学 发酵科学
- 应用微生物学 应用微生物学
背景情况:
- 越来越多的消费者对植物乳制品替代品的需求需要了解它们的微生物特征.
- 与传统发酵乳制品进行比较对于质量和安全性评估至关重要.
研究的目的:
- 研究和比较商业乳制品和植物性发酵奶酪和奶酪的微生物特征.
- 确定植物替代品中的关键微生物差异和潜在的安全问题.
主要方法:
- 经典的微生物学分析.
- 分子方法,包括16S rRNA基因的高通量测序 (HTS),用于细菌和真菌的分析.
主要成果:
- 乳制品和植物性产品都由酵母和乳酸细菌 (LAB) 主导.
- 植物性产品表现出更大的细菌多样性,包括有益的种类和偶尔的腐烂生物,如Raoultella和Pseudomonas.
- 在植物性产品中,Enterococcus物种更为普遍,而Enterobacteriaceae和大肠杆菌只在一些植物性样本中被发现.
- 菌分析显示了变异性,一些植物性产品含有潜在的环境或病原菌,如Cladosporium和Neorysiphe.
结论:
- 乳制品具有一致的发酵驱动的微生物群.
- 植物性发酵产品具有更高的微生物复杂性,需要仔细管理起始培养和加工控制.
- 有针对性的微生物管理对于确保创新的植物乳制品替代品的安全性,功能性和质量至关重要.
相关概念视频
Applications of Molecular Taxonomy
500
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
500
Modern Molecular Taxonomy
574
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
574
Microorganisms in Agriculture and Food industry
1.3K
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
1.3K
The Roles of Bacteria and Fungi in Plant Nutrition
46.5K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
46.5K


