通过真空浸丰富多的豆:在体外结肠发酵过程中增强抗氧化活性和代谢物生物转化
Saúl González-Moya1, Alba Cecilia Durán-Castañeda1, Rita María Velázquez-Estrada1
1Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Laboratorio Integral de Investigación en Alimentos, Av. Tecnológico No 2595, Col. Lagos del Country, Tepic CP, 63175, Nayarit, Mexico.
Food research international (Ottawa, Ont.)
|November 4, 2025
概括
真空浸增强了豆的果多,提高了其肠道发酵和功能性质. 这种方法改善了聚醇与豆矩阵的相互作用,以获得更好的健康益处.
科学领域:
- 食品科学与技术 食品科学与技术
- 营养生物化学 营养生物化学
- 微生物学 微生物学
背景情况:
- 雅姆豆 (Pachyrhizus erosus L.) 富含食纤维,但缺乏多,限制其健康益处.
- 多对于功能性食品至关重要,具有抗氧化和益生菌作用.
- 提高豆等未充分利用的茎的多含量可以增加它们的市场价值和健康影响.
研究的目的:
- 为了评估真空浸 (VI) 用果种子多基提取物对豆体外结肠发酵的影响.
- 评估VI和发酵后的化合物,抗氧化活性和微生物代谢物的变化.
- 确定VI是否增强了多和豆矩阵之间的相互作用,以改善功能性质.
主要方法:
- 果种子提取物是使用超声波辅助提取方法制备的.
- 亚姆豆切片通过真空浸 (VI) (150 mBar,15 分钟) 用果种子提取物处理.
- 被处理和对照的豆样本经过了体外胃肠道消化,随后与人类便微生物群进行了24小时的无氧发酵.
- 分析了化合物,抗氧化活性 (DPPH测定),pH和微生物代谢物 (HPLC-DAD-MS,GC-MS).
主要成果:
- 与对照和非真空处理的样本 (T2) 相比,VI处理 (T1) 显著增加了可溶性和抗氧化活性.
- 在发酵过程中,T1的pH值下降较大,这表明微生物活性增强.
- 通过HPLC-DAD-MS确定了24种化合物,其中T1显示了更高的丰度和更早出现的关键化合物,如酸和酸代谢物 (urolithins).
- GC-MS显示出不同的发酵概况,以为主导T1的早期发酵,脂肪酸则以后.
结论:
- 真空浸是一种有效的策略,可以将果种子中的多纳入豆中,显著提高其功能性质.
- VI过程改善了多-豆矩阵相互作用,导致结肠发酵期间代谢物多样性和度增加.
- 这种方法为开发促进肠道健康的功能性食品提供了一个有希望的途径,通过利用富含多的副产品来促进肠道健康.
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