相关实验视频

Updated: Jan 12, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

在牙发育过程中通过CK2/PER2途径促进循环驱动矿化

Manzhu Zhao^1,2,3,4, Hongyan Yuan^1,2,3,4,5, Di Wang^1,2,3,4

¹College of Stomatology, Chongqing Medical University, Chongqing, China.

Cell proliferation

|November 4, 2025

概括

p75神经受体 (p75NTR) 通过同步与昼夜节律来调节每日牙矿化和发育. 缺少它会破坏这些过程,影响切肢的喷发和生长.

科学领域:

生物化学生物化学
发展生物学发展生物学
时间生物学时间生物学

背景情况:

循环节律调节与光暗周期同步的生理过程.
昼夜节律调节在牙发育中的作用尚未完全理解.
p75神经受体 (p75NTR) 参与各种生物过程.

研究的目的:

调查p75NTR在牙发育过程中的昼夜节律调节中的作用.
探索p75NTR影响日常矿化和牙形态发生的机制.
阐明昼夜节律与牙发育之间的相互作用.

主要方法:

鼠牙细菌的免疫光和时空分析.
在p75NTR淘汰赛小鼠 (p75NTR^ExIII-/-) 中循环节律跟踪.
对基因和蛋白质表达的分析,包括时钟基因,矿化因子和CK2/PER2通路.

主要成果:

在发育大鼠牙时,p75NTR表达与时钟基因和矿化因子同步振荡.
p75NTR^ExIII-/-小鼠表现出延迟的切口喷发,减少矿化,以及改变的时钟基因表达.
p75NTR通过CK2通路影响PER2酸化和核积累,影响牙发育.

结论:

p75NTR对于调节昼夜驱动的矿化和牙形态发生至关重要.
这种p75NTR可能通过CK2/PER2通路调解其作用.
这项研究提供了关于昼夜节律与牙发育之间的关联的关键见解.

关键词:

素酶 2 2 的作用.昼夜节律昼夜节律在p75神经营蛋白受体中,p75神经营蛋白受体酸化的方法是:光化.牙的发育牙的发育

更多相关视频

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

相关概念视频

Hormones and Bone Tissue

Hormones and Bone Tissue

The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...

Skeleton and Calcium Homeostasis

Skeleton and Calcium Homeostasis

Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

The Parathyroid Glands

The Parathyroid Glands

The two pairs of parathyroid glands embedded within the posterior surface of the thyroid gland are restricted by a dense capsule around them. These glands comprise two distinct cell populations—parathyroid oxyphil and parathyroid principal cells- pivotal in calcium homeostasis.
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by...