菌株特异性Bacillus subtilis衍生银纳米颗粒对抗多抗药性病原体具有有效的抗菌活性:在体外模型中
Priyanka Singh1, Ivan Mijakovic1,2
1The Novo Nordisk Foundation, Center for Biosustainability, Technical University of Denmark, Kogens Lyngby, DK-2800, Denmark.
International journal of nanomedicine
|November 4, 2025
概括
野生型Bacillus subtilis 3610菌株高效地生物合成银纳米粒子 (AgNPs) 和金纳米粒子 (AuNPs),与实验室菌株168相比,其抗菌性能得到了增强. 这突出了优化纳米材料生产的菌株特征.
科学领域:
- 纳米生物技术纳米生物技术
- 纳米颗粒的微生物生物合成.
- 对抗微生物的应用.
背景情况:
- 纳米粒子 (NPs),包括白银 (AgNPs) 和黄金 (AuNPs),对于药物输送和稳定性至关重要.
- 可持续和生物相容的NP生产方法有很高的需求.
- 细菌细菌菌株为NP生物合成提供了潜力.
研究的目的:
- 使用两个细菌菌株 (168和3610) 调查和比较AgNPs和AuNPs的生物合成效率.
- 评估生物合成纳米颗粒的抗菌潜力.
- 确定细菌菌株遗传学对NP生产和疗效的影响.
主要方法:
- 使用B. subtilis168和3610超和细胞颗粒的AgNP和AuNP的生物合成.
- 使用UV-Vis,sp-ICP-MS,TGA,SEM,TEM,FTIR,MALDI-TOF,泽塔潜力和AFM进行NP的表征.
- 通过MBC测定,活/死染色和SEM对Pseudomonas aeruginosa和Escherichia coli的抗菌活性的评估.
主要成果:
- 与B. subtilis 168.8相比,Bacillus subtilis 3610表现得更快,更有效地合成了AgNPs和AuNPs.
- AgNP是球形和细胞外产生的,而AuNP是多分散的和与膜相关的.
- 3610衍生的AgNP对P. aeruginosa和E. coli的抗微生物有效性显著提高.
结论:
- 细菌细菌3610具有优越的NP生物合成能力和抗菌功效,超过168.
- 菌株特定的遗传和代谢因素极大地影响微生物纳米材料的生产.
- 从3610衍生出的AgNP显示出生物医学和抗菌应用的前景.
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