在LSD检测方面的进步和挑战:分析技术,矩阵选择和验证差距在法医毒理学中的差距
Diksha Thakur1,2, Neeti Prakash Dubey3, Rajvinder Singh4
1State Forensic Science Laboratory, Karnal, India.
Critical reviews in analytical chemistry
|November 4, 2025
概括
在法医毒理学中检测lysergic acid diethylamide (LSD) 面临的挑战是由于其低剂量和快速代谢. 液体染色学-双重质谱法 (LC-MS/MS) 是最敏感的方法,但需要对矩阵进行标准化验证.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- lysergic acid diethylamide (LSD) 的滥用给公共卫生和法医带来了挑战,并由药物促成的犯罪复杂化.
- 在生物样本中检测LSD及其类型,特别是死后,由于低剂量,快速新陈代谢和与其他lysergamides的结构相似性,很难.
研究的目的:
- 在法医毒理学中批判性地审查和分析用于LSD检测的验证分析方法.
- 确定LSD和代谢物分析当前方法的趋势,挑战和差距.
主要方法:
- 从1978年到2025年进行了一项系统的文献审查,使用PubMed,ScienceDirect和Google Scholar进行.
- 分析了提取技术 (LLE,SPE,DLLME) 和分析平台 (GC-MS/MS,LC-MS/MS,CE-MS) 用于在各种生物矩阵中检测LSD.
- 专注于验证参数,包括灵敏度,特异性,恢复,LOD,LOQ,矩阵效应和稳定性.
主要成果:
- 液体染色学-并联质谱学 (LC-MS/MS) 成为最敏感和广泛验证的技术.
- 对于LSD检测的矩阵特定验证和稳定性评估仍然存在差异.
- 缺乏LSD类型的认证参考材料和依赖于矩阵的降解是重大挑战.
结论:
- 目前的法医LSD检测协议存在关键差距,需要对各种矩阵进行标准化和验证的方法.
- 对现代法医验证指南的有限遵守影响了结果的可复制性和法律可接受性.
- 未来的研究应该专注于快速,环保,高通量方法,用于超微量检测LSD及其类似物.
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