相关实验视频
Updated: Jan 12, 2026
05:50
Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
Published on: June 2, 2023
1.7K
普罗西亚尼丁C1通过RAGEs的表观遗传调节改善了骨关节炎
Xiaoqing Wu1, Rong Xiang1, Lin Yang1
1Department of Orthopaedics and Traumatology, Shenzhen University General Hospital, Shenzhen, 518055, China. sxr0323@sina.com.
Food & function
|November 4, 2025
概括
在水果中发现的Procyanidin C1 (PC1) 化合物,可以通过表观遗传抑制RAGE表达,减少炎症和改善细胞代谢来帮助治疗骨关节炎 (OA).
科学领域:
- 营养科学 营养科学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 先进的糖化终产品 (RAGEs) 的受体上调与骨关节炎 (OA) 病原发生有关.
- 饮食化合物为OA提供了潜在的治疗策略.
- 了解OA中的表观遗传机制对于开发有针对性的干预措施至关重要.
研究的目的:
- 调查普罗西安丁C1 (PC1) 作为OA中的RAGE表达的潜在表观遗传抑制剂.
- 阐明PC1对OA病变发生的保护机制.
- 探索RAGE促进物甲基化在PC1影响中的作用.
主要方法:
- 用PC1.1治疗冠状细胞细胞系和人类初级OA冠状细胞.
- 评估RAGE表达,活性氧物种 (ROS) 生产和NF-κB活性.
- 对冠状体和炎症标志物,线粒体呼吸和糖分水平的分析.
- 研究状细胞亡和自.
- 使用dCAS9-TET1针对RAGE促进体进行验证.
主要成果:
- PC1剂量依赖性降低了红细胞中的RAGE表达.
- PC1抑制了OA胆红细胞中的ROS产生和NF-κB活性.
- PC1通过调节胆固醇/炎症标志物和代谢平衡,改善了OA的微环境.
- PC1 抑制了OA 冠状细胞亡,并促进了自.
- 通过RAGE过度表达,PC1介导的益处被逆转.
- PC1诱导RAGE促进体的高甲基化,这种效应得到了dCAS9-TET1.1的验证.
结论:
- PC1通过通过RAGE促进剂高甲基化对RAGE表达进行表观遗传下调来对OA产生保护作用.
- PC1重新平衡细胞代谢,减少氧化应激和炎症,并促进细胞存活.
- PC1代表了对抗骨关节炎的营养干预策略的有希望的候选人.
相关概念视频
01:20The JAK-STAT Signaling Pathway
11.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
11.9K
01:11Genome-wide Association Studies-GWAS
15.3K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
15.3K
01:37Epigenetic Regulation
3.7K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.7K