母亲血液转录组反映了胎儿生长的变化,是牛出生体重的准确预测器
Laura Thompson1, Pim G van Helvoort2, Maurice Duijn2
1School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
概括
孕产妇血液基因表达分析可以预测牛犊出生体重 (BW). 这种非侵入性方法可以识别胎儿生长监测的关键生物标志物,这些生物标志物可能适用于各种牲畜物种.
科学领域:
- 动物科学动物科学
- 基因组学就是基因组学.
- 生殖生物学 生殖生物学
背景情况:
- 孕产妇血液分析提供了一种非侵入性的方法来监测牲畜中胎儿的发育.
- 了解妊娠期间的时间基因表达变化对于预测胎儿生长结果至关重要.
研究的目的:
- 为了研究母牛妊娠期间母亲血液基因表达的时间变化.
- 确定63日母亲血液转录组修改与小牛出生体重 (BW) 之间的关联.
主要方法:
- 在整个妊娠期内对公开可用的基因表达数据集进行生物信息分析.
- 来自携带体外生产胚胎的奶牛母体血液样本的RNA测序.
- 权重基因共同表达网络分析和差异性基因表达分析.
主要成果:
- 基因表达集群揭示了时间模式,包括干扰素反应,氧化酸化和免疫反应基因.
- 四个同表达模块与小牛的BW相关 (p < 0.05).
- 189个差异表达基因 (DEG) 与小牛体质相关,具有明显的酸化/蛋白质修饰 (积极关联) 和免疫过程 (消极关联) 的丰富.
- 使用妊娠年龄和DEG识别的生物标记基因预测小牛的BW具有高R2值 (0.88和0.75).
结论:
- 对母体血液的转录组分析可以确定可靠的生物标志物,用于预测小牛的出生体重.
- 这些发现表明在畜牧养殖和健康管理方面有潜在的应用.
- 这种方法可以扩展到预测其他动物物种的胎儿生长.
更多相关视频
09:04Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
8.2K
08:26Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
Published on: August 31, 2016
8.4K
相关概念视频
01:14Fetal Circulation
2.5K
Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
2.5K
02:30Genomic Imprinting and Inheritance
36.8K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
36.8K