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Updated: Jul 5, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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通过SrtA介导的结合和循环结合来表现草的体表达

Jun-Hao Xue1, Hui-Min Wei1, Fan Wu1

  • 1School of Life Sciences and Medical Engineering, Anhui University, Hefei 230601, P.R. China.

Organic letters
|November 4, 2025
PubMed
概括

我们开发了一种化学酶方法,以创建新的循环仿真体. 使用这种方法,我们确定了一个强大的双循环depsipeptide,该depsipeptide针对TEAD4,展示了一种构建多样化库的新方法.

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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library07:32

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相关概念视频

Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...