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通过RNA/cDNA混合标记来从低容量总RNA中制备RNA-seq库的协议

Yanling Chen¹, Yukun Hu², Xi Chen²

¹GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou Medical University, Guangzhou, Guangdong 511436, China.

|November 4, 2025

概括

这项研究详细介绍了用于基因表达分析的SHERRY (测序异种RNA-DNA混合) 协议. 它提供了一种可靠且经济的方法,用于使用RNA测序来分析多基化RNA.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

RNA测序 (RNA-seq) 对于基因表达研究至关重要.
现有的方法可能在效率或成本方面存在局限性.
谢里 (测序异种RNA-DNA混合) 提供了一个替代方法.

研究的目的:

为SHERRY图书馆准备一个详细的协议.
为了实现强大的和经济的基因表达量化.
概述RNA净化,逆转录和库生成的步骤.

主要方法:

使用了SHERRY (测序异种RNA-DNA混合) 协议.
从200 ng的总RNA中进行图书馆准备.
包括RNA净化,逆转录,混合标记和库生成的步骤.

主要成果:

描述了为SHERRY图书馆准备一个全面的协议.
该方法可以直接标记RNA/DNA混合体.
为基因表达量化提供了基础.

结论:

谢里协议为基因表达分析提供了一种强大而经济的方法.
提供了图书馆准备,排序和数据分析的详细步骤.
该协议增强了 RNA 测序对多基化 RNA 分析的实用性.

关键词:

基因表达基因表达基因组学就是基因组学.在RNA-seqqq.序列化是指测序的使用.

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相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...