斑点尿是测量可佩丁的合适矩阵
Julie Sherfan1, Sonya Franke1, Myron Lee1
1Chemical Pathology Department, NSW Health Pathology, Liverpool Hospital, Sydney, Australia.
Clinical chemistry and laboratory medicine
|November 4, 2025
概括
尿液是测量可佩的有效矩阵,尿液可佩/肌素比率的衍生参考极限. 尿样在各种条件下保持稳定,支持其在临床诊断中的使用.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 分析化学 分析化学
背景情况:
- 科佩是阿尔金因压素的替代标记物,在各种临床条件下越来越多地被研究.
- 验证尿液作为测量可佩的矩阵对于开发非侵入性诊断工具至关重要.
研究的目的:
- 为了验证尿液作为适合测量可佩的矩阵.
- 为了在健康个体中建立尿可佩/肌素比率参考范围.
- 在不同的分析前条件下评估尿中可佩的稳定性.
主要方法:
- 通过在血清和尿液中使用spiking校准器进行矩阵效应分析.
- 在不同温度下对含有或不含防腐剂的尿样进行稳定性测试.
- 从120名健康志愿者身上推导出尿中可佩/肌素的比率.
主要成果:
- 在健康人群中,尿中的可佩/肌因比为<2.5 pmol/mmol肌因.
- 尿中可佩丁度的基于性别的差异通过肌素校正得到正常化.
- 尿液样本在各种温度 (4-25°C和-20°C) 中显示稳定性7天.
结论:
- 尿液矩阵准确地反映了可佩的动力学,通过BRAHMS Thermo Fisher Copeptin测定验证了其使用.
- 虽然临床效用仍在研究中,但尿中可佩显得有前途,特别是在患有自身性多囊性病的患者中.
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