可注射的阿普他默功能化核酸-原水凝作为血管新生应用的生物活性平台
Nikolaos Pipis1, Dmitry O Traktuev2, Zeyu Liu3
1Ben Towne Center for Childhood Cancer Research, Seattle Children's Hospital, Seattle, WA, 98105, USA.
Biomaterials advances
|November 4, 2025
概括
可注射的核酸原复合体 (NACCs) 水凝通过激活VEGFR-2促进新的血管形成. 这种生物相容材料支持再生医学应用,没有系统性炎症.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 血管生物学 血管生物学
背景情况:
- 核酸原复合体 (NACC) 是使用I型原和单链DNA (ssDNA) 的自组合生物材料.
- 血管新生,新血管的形成,对于组织修复和再生至关重要.
研究的目的:
- 开发可注射的NACCs,其功能与血管内皮生长因子受体2 (VEGFR-2) 激活aptamer促进局部血管生成.
- 评估这些工程NACC的稳定性,可注射性,体外生物活性和体内疗效.
主要方法:
- 通过自身组装的原和ssDNA制造NACCs,功能化与VEGFR-2的aptamer.
- 在富含核酶的环境中评估阿巴胺稳定性和用于注射的水凝类风学.
- 在体外研究使用内皮细胞在3D培养中评估细胞行为和血管结构的形成.
- 在小鼠体内进行皮下植入,通过细胞因子分析评估宿主细胞透,组织重塑,血管生成和全身炎症.
主要成果:
- 在NACC中的VEGFR-2胺基仍然不受降解的影响,而水凝对25G针的注射性表现出剪切稀释特性.
- 在体外,NACCs支持内皮细胞的附着,扩散,增殖和血管类结构的形成.
- 在体内,植入的小鼠显示出显著的宿主细胞透,组织重塑和强大的新血管形成,没有外源因素.
- 观察到最小的系统性炎症,血中细胞因子水平低或无法检测,证实了生物相容性.
结论:
- ssDNA 胺酶功能化的 NACCs 形成一个生物活性,可注射的水凝平台,用于促进血管生成.
- 该平台通过支持亲血管性重塑和表现出出色的生物相容性,证明了再生医学的翻译相关性.
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