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Updated: Jan 12, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
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Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

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在非重复性内源性位点对DNA损伤反应的时间过程的成像

Adam T Rybczynski1, W Taylor Cottle2, Po-Ta Chen3

  • 1Howard Hughes Medical Institute and Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA; Department of Biology, Johns Hopkins University, Baltimore, MD, USA.

Cell reports methods
|November 4, 2025
PubMed
概括

研究人员开发了一种新方法来追踪DNA双链断裂 (DSB) 修复动态. 这项技术使用CRISPR和GOLDFISH以高时间分辨率可视化修复因子,如γH2AX,53BP1和BRCA1.

关键词:
CP:成像成像的使用.科普:分子生物学我们的DNA是DSB的DNA鱼的DNA 鱼的DNA对DNA损伤的反应反应对DNA损伤反应的动力学DNA 双链断裂发生修复DNA的修复DNA的修复黄金鱼是一种金鱼.vfCRISPR 的使用情况.

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