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相关概念视频

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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无散射UV可见光谱技术用于精确和精确的RNA量化复杂RNA纳米粒子配方中的RNA量化.

Aida López Espinar1, Eric C Le Ru2,3, Parveen Kumar1

  • 1School of Pharmacy, University College Cork, Cork T12 K8AF, Ireland.

Analytical chemistry
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概括

在复杂的纳米粒子药物中准确测量核糖核酸 (RNA) 是很困难的. 无散射吸收光谱 (SFAS) 提供了一种可靠的方法来量化完整的纳米粒子中的总RNA,其性能优于传统的光分析.

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科学领域:

  • 生物材料科学 生物材料科学
  • 分析化学 分析化学
  • 制药科学 制药科学

背景情况:

  • 基于核糖核酸 (RNA) 的疗法对治疗各种疾病具有前景.
  • 有效的临床应用需要复杂的纳米粒子 (NP) 配方.
  • 在这些复杂的NP中精确量化总RNA是一个重要的分析挑战.

研究的目的:

  • 评估无分散吸收光谱法 (SFAS) 作为复杂的RNA载荷纳米粒子中总RNA量化的方法.
  • 将SFAS的性能与已建立的光测试进行比较 (RiboGreen,SYTO 9).
  • 为了证明SFAS在各种NP配方中的实用性,耐扰.

主要方法:

  • 使用无散射吸收光谱法 (SFAS),一种UV/可见技术,旨在消除NP组件的光散射.
  • 在各种RNA配方上测试了SFAS:脂质NP,聚合物/树脂分子混合脂质NP和环极素纳米复合物.
  • 将SFAS结果与使用RiboGreen和SYTO 9染料的光分析进行比较.

主要成果:

  • 在所有测试的NP配方中,SFAS提供了准确,精确和可重复的总RNA量化.
  • SFAS超越了基于光的方法,特别是对于具有抗破坏性NP的NP.
  • 通过SFAS对RNA的量化显示,NP的组成和测量条件对NP的影响很小.

结论:

  • SFAS是一种基于光测试的多功能和可靠的替代方法,用于在复杂的RNA NP配方中量化总RNA.
  • 这种方法可以在完整的NP中准确测量RNA,简化配方分析.
  • SFAS为基于RNA的纳米药物的质量控制和开发提供了一个强大的解决方案.