无散射UV可见光谱技术用于精确和精确的RNA量化复杂RNA纳米粒子配方中的RNA量化
Aida López Espinar1, Eric C Le Ru2,3, Parveen Kumar1
1School of Pharmacy, University College Cork, Cork T12 K8AF, Ireland.
Analytical chemistry
|November 4, 2025
概括
在复杂的纳米粒子药物中准确测量核糖核酸 (RNA) 是很困难的. 无散射吸收光谱 (SFAS) 提供了一种可靠的方法来量化完整的纳米粒子中的总RNA,其性能优于传统的光分析.
科学领域:
- 生物材料科学 生物材料科学
- 分析化学 分析化学
- 制药科学 制药科学
背景情况:
- 基于核糖核酸 (RNA) 的疗法对治疗各种疾病具有前景.
- 有效的临床应用需要复杂的纳米粒子 (NP) 配方.
- 在这些复杂的NP中精确量化总RNA是一个重要的分析挑战.
研究的目的:
- 评估无分散吸收光谱法 (SFAS) 作为复杂的RNA载荷纳米粒子中总RNA量化的方法.
- 将SFAS的性能与已建立的光测试进行比较 (RiboGreen,SYTO 9).
- 为了证明SFAS在各种NP配方中的实用性,耐扰.
主要方法:
- 使用无散射吸收光谱法 (SFAS),一种UV/可见技术,旨在消除NP组件的光散射.
- 在各种RNA配方上测试了SFAS:脂质NP,聚合物/树脂分子混合脂质NP和环极素纳米复合物.
- 将SFAS结果与使用RiboGreen和SYTO 9染料的光分析进行比较.
主要成果:
- 在所有测试的NP配方中,SFAS提供了准确,精确和可重复的总RNA量化.
- SFAS超越了基于光的方法,特别是对于具有抗破坏性NP的NP.
- 通过SFAS对RNA的量化显示,NP的组成和测量条件对NP的影响很小.
结论:
- SFAS是一种基于光测试的多功能和可靠的替代方法,用于在复杂的RNA NP配方中量化总RNA.
- 这种方法可以在完整的NP中准确测量RNA,简化配方分析.
- SFAS为基于RNA的纳米药物的质量控制和开发提供了一个强大的解决方案.
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