在空气-液体界面上吸附的单克隆抗体的剪切和扩展性风湿学和界面结构
Kiet G Pham1, Benjamin R Thompson1, Minh Phan1,2
1Department of Chemical & Biomolecular Engineering, Center for Neutron Science, University of Delaware, Newark, Delaware 19716, United States.
Langmuir : the ACS journal of surfaces and colloids
|November 4, 2025
概括
蛋白质在空气-液体界面形成粘弹性薄膜,影响生物制药的稳定性. 这项研究揭示了单克隆抗体 (mAb) 膜中的高界面弹性与β片结构有关,为配方开发提供了洞察力.
科学领域:
- 表面科学和合体化学
- 生物物理化学 生物物理化学
- 材料科学是一种材料科学.
背景情况:
- 蛋白质充当两性动物,在疏水性空气液体接口上吸附,形成粘弹性薄膜.
- 表面粘弹性对于发泡性,乳液稳定性和生物制药配方稳定性至关重要,特别是对于单克隆抗体 (mAbs).
- 在mAb配方中的界面聚合和剪切弹性与长期稳定性相关.
研究的目的:
- 通过使用新型界面风力计,研究吸附单克隆抗体 (mAb) 界面薄膜的扩张和剪切风力学.
- 探索 mAb 界面膜的结构-神经学特性,包括以前未被研究的"堵塞"状态.
- 为了确定接口风学与实际表面覆盖率之间的关系.
主要方法:
- 利用新的界面风力计来测量吸附的mAb膜的膨胀和剪切风力学.
- 采用X射线反射率 (XRR) 来进行外平面结构分析和确定表面过量.
- 使用布鲁斯特角显微镜 (BAM) 进行平面内结构信息和薄膜均性评估.
主要成果:
- mAb界面膜主要表现出弹性 (类似固体) 的行为,在压缩时2D波桑比率从0.9降至0.4.
- BAM证实了薄膜的均性,而XRR则在空气接口上发现了一层缩的蛋白质层.
- 计算了2.54.0 Å的局部长度 (r_loc),支持了部分mAb展开导致高界面弹性的β-sheet结构的假设.
结论:
- mAb膜的高界面弹性归因于β-sheet结构的形成.
- 这项研究确定了界面形学和测量表面覆盖率之间的缩放关系.
- 这些研究结果为吸附的mAb层的结构-神经学性质提供了基本的见解,这与生物制药应用有关.
相关概念视频
02:57Surface Tension, Capillary Action, and Viscosity
28.6K
Surface Tension
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
28.6K
01:25Selectins
3.6K
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.6K
01:31Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
01:18Surface Membrane Barriers
3.0K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
3.0K
01:26Antibody Actions
3.9K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
3.9K
01:29Adsorption Isotherms I
235
Adsorption isotherms are mathematical models that describe how molecules in a gas or liquid phase interact with surfaces. Two of the most common isotherm models are the Langmuir and Freundlich isotherms, which relate to Type I monolayer chemisorption. The Langmuir model is based on four key assumptions:• Adsorption cannot exceed monolayer coverage.• All surface sites are equivalent.• Molecules adsorb only at vacant sites.• There are no interactions between adsorbed...
235